Thermo Fisher Krios Details

Location: Piscataway, NJ at the Rutgers CryoEM & Nanoimaging Facility (RCNF). Director: Prof. Jason Kaelber

The 300kV Krios TEM is optimized for high-throughput, high-resolution imaging for single particle cryoEM and tomography applications. 

This instrument is available to anyone. Please contact Mariena for more info on how to access this tool. 

• A high-brightness Schottky electron source (X-FEG) is used for high image resolution
• 300kV provides a longer mean free path enabling imaging of thicker specimens
• Autoloader allows up to 12 grids to be loaded under liquid nitrogen and kept under vacuum ready for imaging
• Automatic liquid nitrogen filling allows unattended operation
• Stable stage for low-drift imaging at cryogenic conditions and capable of high tilt for tomography (+/- 70° tilt)
• K3 direct electron detector for high-quality TEM imaging
• Gatan BioQuantum energy filter: zero-loss filtered imaging for increased contrast, especially in thicker specimens
• Automated data collection for single particle EM and tomography using EPU or SerialEM software
• Available modes of operation: single-particle cryoTEM, cryo-electron tomography, and microED