WITec Alpha300R Confocal Raman Microscope
WITec Alpha300R Confocal Raman Microscope Details
Bard Hall B56 (Primary Staff: Kevin Silverstein; Secondary Staff: Mark Pfeifer)
The Alpha300R can be used to investigate existing chemistry, track chemical bonds through a process, investigate crystallinity, image strain fields, and measure photoluminescence. Minimum sample volumes of less than a µl are possible since the analysis is taking place through a microscope. Thin films are very common and in some cases monolayers can be measured.
The Alpha300R incorporates 2 laser excitation sources (532 nm and 785 nm), an advanced confocal microscope, 2 high throughput spectrometers and software capable of powerful multivariate analysis into a system that is built around spectral mapping. See the example below for more information on spectral mapping.
Specifications
Spatial Resolution:
- X, Y (in focal plane): ~300 nm depending on laser excitation
- Z (normal to focal plane): ~800 nm depending on objective numerical aperture and laser excitation
Diffraction Gratings:
- For use with 532 nm laser:
- 300 l/mm
- 1200 l/mm
- 1800 l/mm
- For use with 785 nm laser:
- 300 l/mm
- 1200 l/mm
Spectral Resolution
- ~3 cm-1 with 300 l/mm
- ~0.5 cm-1with 1200 l/mm
- ~0.1 cm-1 with 1800 l/mm
Required Concentrations:
- In general, concentrations of samples need to be on the order of ~parts per thousand to acquire a spectrum with a good signal to noise ratio.
Available Objectives:
Standard
- 5x
- 20x; 0.50 N.A.
- 50x LWD; 0.50 N.A.
- 100x; 0.90 N.A.
Non-standard
- 20x dipping
- 40x dipping
- 63x dipping
- 63x oil immersion; 1.4 N.A.
- 100xLWD
