Thanks to our sponsors!
Congratulations to the poster presenters and to the image awardees!
The 2016 CCMR Symposium took place on May 25 on the Ithaca campus. It gathered 158 attendees from industry and academia. Representatives of 11 academic institutions and 25 companies networked with Cornell faculty and Symposium speakers and learned more about the electron microscopy strengths of Cornell University. Cornell Applied Physics and Engineering Physics Professors Lena Kourkoutis and David Muller welcomed Professor Joachim Frank (Columbia University) who gave the 2016 Sproull Lecture; he was joined by Dr. Richard Leapman, Chief of the Laboratory of Cellular Imaging and Macromolecular Biophysics at the National Institute of Biomedical Imaging and Bioengineering (NIBIB); Professor Darrin Pochan, Materials Science and Engineering, University of Delaware; Professor Rafal Dunin-Borkowski, Director of the Institute for Microstructure Research and the Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons, Forschungszentrum Jülich, Germany; Prof. Philip Batson, Physics and Astronomy, Rutgers, and by Megan Holtz, Graduate Student, Applied and Engineering Physics, Cornell University.
Professor Joachim Frank focused on the mechanism of translation on the ribosome and gave a historical perspective of single-particle cryo-electron microscopy, which today provides near-atomic resolution information about the 3D structure of biological molecules. Successful realization of the single-particle approach with frozen-hydrated samples (hence, single-particle cryo-EM) required the convergence of three fundamental technical developments: specimen preparation, electron microscopy and computational approaches for data processing. The art of specimen preparation was revolutionized by the development of plunge freezing, a technique which ensures that molecules be rapidly frozen and suspended in a thin layer of ice. On the instrument side, there were efforts to understand the mechanism of image formation, and to improve the performance of the transmission electron microscope tailored to the needs of low-dose imaging of frozen-hydrated specimens. Mathematical concepts and computational procedures were developed for recovering structures of molecules lacking symmetry and order. One of the most promising aspects of single-particle cryo-EM is the ability of the technique to visualize multiple states, all coexisting in the same sample. The ribosome provided an interesting case studies as it goes through a cycle of binding events and conformational changes for each amino acid to be added to the growing polypeptide. Atomic structures have now come into view, and single-particle cryo-EM has become a serious competitor of X-ray crystallography.
The lectures also illustrated also the value of cryogenic TEM to analyze and design nanostructure with polymer and peptides, as well as to observe electrochemical processes in batteries and fuel cells, map electromagnetic fields and provide atomic level analysis of composition, structure and function of nanoscale semiconductor devices.
CCMR Symposium Poster Session
The Symposium off-campus attendees selected for best posters:
(* Principal Investigator)
The attendees selected for best images:
The following day (May 26) GATAN held an EELS and EFTEM workshop focusing on the beginner and advanced practice of EELS imaging and analysis in the (S)TEM using a variety of EELS techniques. Following GATAN’s lecture, 30 participants from Cornell, industry and other academic institutions, had the opportunity to participate in comprehensive, hands-on training on advanced EELS and EFTEM topics. The practical sessions on the microscope were given using Cornell’s Cs-corrected FEI Titan Themis equipped with a GIF Quantum ER.
Organizing Committee: Profs. Lena Kourkoutis and David Muller, Applied and Engineering Physics.
GOLD SPONSOR: FEI North America
GOLD SPONSOR: Corning Inc.